Document Type : Original Article
Authors
1 Hazrat-e Masoumeh University
2 Department of Textile Engineering, Amirkabir University of Technology, 15875-4413, Tehran, Iran.
Abstract
Since poly(lactide-co-glycolic acid) (PLGA), as a biodegradable material, is a hydrophobic polymer which might lead to the incoherence of optimal growth of cells on the scaffold, the scaffold surface modification can promote the cell growth and proliferation. In this study, two methods including structural modification and plasma treatment were employed to improve the surface properties and epithelial kidney cells (Vero) culture efficiency for the PLGA nanofibrous scaffolds. Moreover, the physical, and chemical properties of the modified scaffolds were characterized. Plasma treatment enhances surface hydrophilicity and structural modification improves physical properties of surface such as fiber diameter, surface porosity and alignment index. It was found that the plasma-treated scaffold is more hydrophilic compared to the structurally-modified and non-treated scaffolds. From the ATR-FTIR spectra of the samples, it was observed that the extent of C=O and C-O groups was increased in the plasma-treated samples in comparison with the other groups. Furthermore, in-vitro studies demonstrated that, despite the greater hydrophilicity of the plasma-treated scaffold, both of modified scaffolds enhanced the cell growth and proliferation of Vero cells. In conclusion, the structurally-modified scaffolds have shown a promising potential to improve the cell proliferation as compared with the plasma-modified scaffolds.
Keywords
Nanomaterials, vol. 9, pp. 637-656, 2019. [9] H. Rauscher, M. Perucca, and G. Buyle, Plasma Technology For Hyperfunctional Surface, Weinheim: Weily-VCH, 2010, pp. 63-78. [10] R. Morent, N. De Geyter, T. Desmet, P. Dubruel, and C. Leys, “Plasma surface modification of biodegradable polymers: a reviewˮ, Plasma Proc. Polym., vol. 8, pp. 171-190, 2011. [11] G.H. Ryu, W.S. Yang, H.W. Roh, I.S. Lee, J.K. Kim, G.H. Lee, D.H. Lee et al., “Plasma surface modification of poly(D, L-lactic-co-glycolic acid) (65/35) film for tissue engineeringˮ, Surf. Coat. Technol., vol. 193, pp. 60-64, 2005. [12] A. Solouk, B.G. Cousins, H. Mirzadeh, and A.M. Seifalian, “Application of plasma surface modification techniques to improve hemocompatibility of vascular grafts: a review”, Biotechnol. Appl. Biochem.,vol. 58, pp. 311-327, 2011. [13] A. Solouk, B.G. Cousins, H. Mirzadeh, M. SolatiHashtjin, S. Najarian, and A.M. Seifalian, “Surface modification of POSS-nanocomposite biomaterials using reactive oxygen plasma treatment for cardiovascular surgical implant applications”, Biotechnol. Appl. Biochem., vol. 58, pp. 147-161, 2011. [14] Z. Liu, L. Jia, Z. Yan, and L. Bai, “Plasma-treated electrospun nanofibers as a template for the electrostatic assembly of silver nanoparticles”, New J. Chem., vol. 42, no. 13, pp. 1-7 , 2018. [15] N. Hasirci, T. Endogan, E. Vardar, A. Kiziltay, and V. Hasirci, “Effect of oxygen plasma on surface properties and biocompatibility of PLGA films”, Surf. Interface Anal., vol. 42, pp. 486-491, 2010. [16] M. Khorasani, H. Mirzadeh, and S. Irani, “Plasma surface modification of poly(L-lactic acid) and poly(lactic-co-glycolic acid) films for improvement of nerve cells adhesion”, Radiat. Phys. Chem., vol. 77, pp. 280-287, 2008. [17] K.E. Park, K.Y. Lee, S.J. Lee, and W.H. Park, “Surface characteristics of plasma-treated PLGA nanofibers”, Macromol. Symp., pp. 103-108, 2007. [18] L. Safinia, K. Wilson, A. Mantalaris, and A. Bismarck, “Through-thickness plasma modification of biodegradable and nonbiodegradable porous polymer constructs”, J. Biomed. Mater. Res., vol. 87A, pp.
632-642, 2008. [19] H. Cao, T. Liu, and S. Chew, “The application of nanofibrous scaffolds in neural tissue engineering”, Adv. Drug Deliver. Rev., vol. 61, pp. 1055, 2009. [20] G. Kim, J. Park, and S. Park, “Surface-treated and multilayered poly(e-caprolactone) nanofiber webs exhibiting enhanced hydrophilicity, J. Polym. Sci: Polym. Phys., vol. 45B, pp. 2038-2045, 2007. [21] L. Huang, J.T. Arena, S.S. Manickam, X. Jiang, B.G. Willis, and J.R. McCutcheon, “Improved mechanical properties and hydrophilicity of electrospun nanofiber membranes for filtration applications by dopamine modification, J. Membrane Sci., vol. 460, pp. 241249, 2014. [22] F. Zamani, M. Amani-Tehran, A. Zaminy, and M.A. Shokrgozar, “Conductive 3D Structure nanofibrous scaffolds for spinal cord regeneration”, Fiber. Polym., vol. 18, pp. 1874-1881, 2017. [23] F. Zamani, M. Latifi, M. Amani-Tehran, and M.A. Shokrgozar, “Effects of PLGA nanofibrous scaffolds structure on nerve cell directional proliferation and morphology”, Fiber. Polym., vol. 14, pp. 698-702, 2013. [24] S. Ramakrishna, K. Fujihara, W.E. Teo, T.C. Lim,
and Z. Ma, An Introduction to Electrospinning and Nanofibers, World scientific, Singapore, 2005, pp. 90155. [25] L. Safinia, N. Datan, M. Hohse, A. Mantalaris and A. Bismarck, “Towards a methodology for the effective surface modification of porous polymer scaffoldˮ, Biomaterials, vol. 26, pp. 7537-7547, 2005. [26] D.L. Pavia, G. Lampman, and G.S. Kriz, Introduction to Spectroscopy, 5rd ed, Cengage Learning, Washington, 2013, pp. 14-106. [27] F. Zamani, “Engineering of structural properties of PLGA nanofbrous scaffold for neural cell cultureˮ, Ph.D Dissertation, Dept. Text. Eng., Amirkabir University of Technology, Tehran, Iran, 2013. [28] GE. Adams, A. Breccia, EM. Fielden, and P. Wardman, Selective Activation of Drugs by Redox Processes, New York: Plenum, 1990, pp. 200-210.
)